Consort Horizontal Electrophoresis Units
Product Details:

Usage/Application	Laboratory
Model Name/Number	EHS1500
Brand	Consort
Buffer Volume	1400 ml
Gel Dimensions	26x16 cm, 26x24 cm, 26x32 cm
Unit Dimensions	50x28x9 cm
Warranty	12 Months

Buffer
TAE buffer provides optimal resolution of fragments >4 kb in length, while for 0.1 to 3 kb fragments, TBE buffer should be selected. TBE has both a higher buffering capacity and lower conductivity than TAE and therefore should be used for high-voltage electrophoresis. Additionally, TBE buffer generates less heat than TAE at an equivalent voltage and does not allow a significant pH drift. Note: because of its lower buffering capacity, TAE should be circulated or mixed from time to time for full-length electrophoresis, especially at higher voltages.
Low cost
Injection moulded construction
Durable, leak-proof environment for complete safety and long life
Cassette type electrodes
Inexpensive, easy to replace. Made of 99.99% corrosion resistant, pure platinum.
Multiple gel trays
Gel size and sample number requirements can be exactly matched in each unit, with the option of additional gel tray sizes.
This eliminates the need for multiple gel tanks for changes in gel size or application. With no indentations or casting gate grooves in the tray to interfere with sample progression, traditional tape casting can be used, should this be preferred.
Easy to use
Leak proof “Plug and Go” casting dams allow gels to be rapidly cast externally while the tank remains in use for electrophoresis.
Gel concentration
The range of fragment sizes to be separated will determine the choice of agarose concentration for a gel. Typical agarose concentration is 0.5% to 3.0%. For large DNA fragments low-percentage gels are required, while for small DNA fragments, high-percentage gels are recommended. Weak gels (0.5% agarose) should be electrophoresed at low temperatures (e.g. -4°C). Agarose gels of 0.75% to 1.0%, for routine electrophoresis, are recommended for a wide range of separations (0.15 to15 kb). 2…4% agarose gels are usually selected for PCR fragment resolution. If the gel has to be subsequently photographed, thin gels (2 to 3 mm) with low-percentage agarose are better than thick or high-percentage gels. The latter produce increased opaqueness and autofluorescence.
Electrophoresis buffer
TAE buffer provides optimal resolution of fragments >4 kb in length, while for 0.1 to 3 kb fragments, TBE buffer should be selected. TBE has both a higher buffering capacity and lower conductivity than TAE and therefore should be used for high-voltage electrophoresis. Additionally, TBE buffer generates less heat than TAE at an equivalent voltage and does not allow a significant pH drift. Note: because of its lower buffering capacity, TAE should be circulated or mixed from time to time for full-length electrophoresis, especially at higher voltages.
Temperature influence
Electrophoresis at high voltages produces heat. Additionally, high-conductivity buffers such as TAE generate more heat than low-conductivity buffers. Care should be taken in agarose gel electrophoresis with voltages greater than 175 V, as heat build up can generate gel artifacts such as S-shaped migration fronts, and in extended electrophoresis runs, can even melt the agarose gel. With high voltage electrophoresis, the use of low-melting-point agarose gels should be avoided.
RNA mobility
Either before or during electrophoresis, RNA should be denatured. For example, RNA fragments which have denatured with glyoxal and dimethyl sulphoxide can be separated on neutral agarose gels, or RNA can be fractionated on agarose gels containing methylmercuric hydroxide or formaldehyde. RNA samples usually require longer runs or buffers that are easily depleted, so it is necessary to circulate the buffer. Northern analyses should not normally be run on a mini gel tank.
Enhancing resolution