End-Point Chromogenic Endotoxin Test Kit (Without Diazo Coupling)

1. Product Information

End-point Chromogenic Endotoxin Test Kit (without Diazo Coupling) is conducted by adding a colorless artificial peptide substrate solution into mixture of Lyophilized Amebocyte Lysate and test sample after a certain incubation period. If test sample contain endotoxin, a yellow color will develop. Its absorbance (λmax = 405nm) is related to the endotoxin concentration. Endotoxin concentration of test sample could be calculated based on a standard curve.

2. Product parameter

Sensitivity Range: 0.01-0.1EU/ml: assay time about 46 minutes

0.1-1EU/ml: assay time about 16 minutes 

3. Product Feature and Application

End-point Chromogenic Endotoxin Test Kit (without Diazo Coupling) is intended for use in the In Vitro detection and quantitation of gram-negative bacterial endotoxins. The colorless artificial peptide substrate solution is added into the mixture of Lyophilized Amebocyte Lysate and test sample after a certain incubation period. Yellow color develops if test sample contains endotoxin. Then use regular spectrophotometer or plate reader to read the absorbance at 405nm. End-point Chromogenic Endotoxin Test Kit (without Diazo Coupling) is suitable for all kinds of biomedical samples such as protein, vaccine, plasmid, DNA, RNA endotoxin assay. 

Note:

Lyophilized Amebocyte Lysate (LAL) reagent manufactured by Bioendo is made from amebocyte lysate derived blood of horseshoe crab.