S.No | Features | Specifications |
1. | A Complete HPLC System | A complete HPLC System with Pumps, columns, oven, PDA, FLD and RI detector. All units must be from a single manufacturer. Technical bids with compatible modules from another manufacturer will not be evaluated. |
2. | Pumps | ·Pump should provide error-free programming of pump parameters including flow rates, operating pressure limits, compressibility compensation, calibration and diagnostics.
·The Solvent Delivery Unit (Pump) should be capable of operating with 2 or more Solvents at a time during gradient operation limits, compressibility compensation, calibration and diagnostics.
·Number of solvent channels should be four.
· Settable flow range should be from 0.001 – 10 ml/min, in 0.001 ml/min increments.
·Flow precision value should be ≤0.07 % RSD.
·Flow accuracy should be ± 1 %.
·Must deliver a flow rate of up to 10mL/min.
· Must have an operation pressure range of 0 – 600 bar.
· Should have a pH range 1.0 — 12.5.
·Integrated degassing unit for 4 channels.
·Composition precision should be < 0.2 % RSD.
·Should have the provision for complete upgradation capability (module wise and system wise) in future. |
3. | Vacuum Degassing
Unit | Online Degassing Unit should have minimum 4 number of flow lines/channels. |
4. | Auto Sampler Capacity With Sample Cooler | ·The Auto sampler must have a capacity to hold 100 samples.
·Must have an operation pressure range of 0 – 600 bar.
·Injection range should be 0.1 to 100 µL.
·Sampler should be capable of injection volume range upto 900uL or higher with additional loop and hardware modification when required.
·Precision should be <0.25 % RSD.
·Carry over should be <0.004 %.
·Injection cycle time should be 20 s or better.
·Should have advanced features like auto dilution, premixing and needle rinsing programs.
·Sampler temp: 4-40 deg. |
5. | Analytical Columns | ·Columns – 5
C18 Analytical column (4.6 mm ID x 250 mm length, particle size 5µm)
C18 Analytical column (3 mm ID x 150 mm length, particle size 2.7µm)
C8 Analytical column (4.6 mm ID x 250 mm length, particle size 5µm)
C18 Analytical column (4.6 x 100 mm, 3 µm)
Silica Analytical column ((4.6 mm ID x 250 mm length, particle size 5µm) |
6. | Column Oven | ·Temperature range: 5 °C above ambient to 80 °C or higher
·Heat-up time: 20-40deg in 5mins or better
·Capacity: Should be able to hold atleast 2x 300mmx 4.6 mm id columns.
·Should be equipped with column pre-heaters
·Temperature stability: ±0.1deg or better |
7. | Photo Diode Array Detector | ·Wavelength range should be 190 – 800 nm or more.
·Detection type should be with 1024-element photodiode array.
· Light source – Deuterium and tungsten lamps.
·Data rate should be up to 100 Hz or better
·Wavelength accuracy = ± 1 nm.
· Slit width should be programmable as 1 or 1.2, 2, and 8 nm.
· Flow cell- Standard: <14 μL volume, 10 mm cell path length
·Short term noise < ± 1·10-5 AU
·Drift < 0.9·10-3 AU/h at 254 nm.
·Linear absorbance range > 2 AU (5 %) at 265 nm.
·Data traceability for flow cells and lamps should be there. |
8. | Fluorescence Detector | ·Light source: Xenon lamp Longer Life (> 2000 hrs).
·Excitation Wavelength range: 200 nm to 800 nm or better
·Emission Wavelength Range: 210-850 nm or better
·Spectral bandwidth: 20 nm
·Wavelength accuracy: ± 3 nm or better
· Wavelength repeatability: ± 0.25 nm or better
·Signal to Noise ratio for Water Raman peak should be > 500.
·Flow cell volume: 8µL. |
9. | Refractive Index Detector | ·Light source: LED source
·RI Range: 1.00 to 1.75. RIU
·Measurement range :±600·10-6 RIU
·Noise level should be < 2 x 10-9 RIU
·Drift: 2 x 10-7 RIU/h
·Compatible with flow rates upto 5 ml/min
·Flow cell volume: 9µL or lower
·Safe leak detection
·Temperature control 5deg above ambient-55 °C or better
·Data collection rate: 60Hz or better
·Automatic purge and automatic solvent recycle valves should be available. |
10. | Chromatography Software | ·Windows based software for evaluation of data, control and operation of entire system.
·System security to be provided by the software and not by the operating system
·User accounts, user privileges and audit trails are maintained by the software and not by the operating system
·Compliance with GMP/GLP ,Electronic signature & Electronic Record rules
·Software should have its own built in report.
·Data that has been reviewed and approved may be locked to prevent further manipulations
·Software should offer extensive custom calculation capabilities (Concentration, calibration curve, peak soothing, peak subtraction etc.) eliminating the need for external applications to meet the laboratory need. |
11. | Computer & Printer & Power Backup | ·Processor type: Intel Core i7 Processor, 8 GB RAM
·Memory size: 1 TB HD, CD-ROM or DVD + RW drive, USB port.
·Operating system; Windows 10 Professional (64 bit) or most recent with license.
·Monitor type: Wide Screen Monitor (Minimum 21”)
·Suitable colour dual side laser printer Software supports for the equipment.
·3 KVA UPS with 60 minutes back up should be provided. |
12. | Accessories | 1.Sample Vials 100 numbers with 1.5 ml or greater.
2.Ultrasonic bath:
I. Stainless Steel Ultra Sonic bath with the capacity of 5 L or more,
II. Time setting (min) 1-30min or continuous operation with LED and Push button (Should be IP 33 Protection class) for sonication of spare parts as well as solvents.
Mobile Phase Filter Assembly
I. Mobile phase filter assembly (2 L) for aqueous and organic solvent
II. Aqueous and organic solvent compatible membranes 0.22 microns 100 numbers each
1.Oil free vacuum pump (1 no.) with 4 bar pressures or better should be Neoprene diaphragm based.
2.Fittings, Frits, ferules and Tubing’s
3.Standards for HPLC Calibration for PDA (caffeine/propylparabens/acetone), RI and Fluorescence detector
4.HPLC solvents Acetonitrile and methanol: 4L each
5.One Spare lamp for PDA. |
13. | Delivery | Delivery till F.O.R Destination. |
14. | Instrument performance | ·Installation at our site with detailed demonstration of all performance specifications including repeatability, software features, do’s and don’ts etc. |
15. | IQ/ OQ | ·IQ, OQ, PQ of instrument and Software should be provided along with document. |
16. | On-site training | ·Site Training for 2-3 working days on operation and troubleshooting should be part of the installation and commissioning of the system |
17. | Warranty | · One year warranty from the date of installation.
· The vendor should have availability for ten years guaranteed parts and services.
· Current user’s / performance list in India of the quoted Model with contact details (Customer name, phone email id etc) and date of installation to be provided (Minimum 5 installations of the model quoted)and this will be taken very seriously while making the decision. Your post sales service feedback will be certainly a deciding factor. |
18. |
Compliance Statement | The supplier must submit technical brochures and proper application notes adequately explaining and confirming the availability of the features in the model of the equipment being quoted.
The supplier must submit a table indicating the compliance of the features of the model of the equipment being quoted with those given in the indent.
Features not matching – must be clearly indicated.
Additional features and features in the quoted equipment which are better than those in the indent – may be clearly explained.
Bids submitted without Technical specifications manuals of each module quoted will not be evaluated for technical compliance.
All pre-installation requirements must be attached. |
Asian Scientific Industries (Delhi) 
