Advantage Malaria Card

Product Details :

Intended Use:

• Advantage Malaria Card is a visual, rapid and sensitive immunoassay for the qualitative differential detection of P.falciparum (HRP-2) and P.vivax (pLDH) malaria antigen in human blood only.

Salient Features:

• Rapid, visual antigen based test for detecting infection with P.falciparum (HRP-2) & P.vivax (pLDH) malaria parasite in whole blood.
• Excellent Sensitivity & Specificity.
• See through Device for easy result interpretation.

Principle:

• Advantage Malaria Card is an immunoassay based on the "Sandwich principle". Colloidal gold is conjugated to P.f. specific monoclonal anti-HRP-2 antibody and monoclonal anti-pan specific pLDH (plasmodium lactate dehydrogenase) antibody. The test uses monoclonal anti-Pf HRP-2 antibody (test line F) & monoclonal anti-P.v specific pLDH antibody (test line V) immobilized on a nitrocellulose strip. The test sample is added to the device. On addition of assay buffer, the red blood cells get lysed. If the sample contains P.falciparum or P.vivax or both, the colloidal gold conjugate complexes the HRP-2 / P.vivax specific pLDH in the lysed sample. This complex migrates through the nitrocellulose strip by capillary action. When the complex meets the line of the corresponding immobilized antibody, the complex is trapped forming a purplish pink band which confirms a reactive test result. Absence of a coloured band in the test region indicates a negative test result. To serve as a procedural control an additional line of anti-mouse antibody has been immobilized on the strip as control.