Agricultural Solutions
Product Details:
Next generation sequencing (NGS) methods allow the rapid and high throughput sequencing of large stretches of DNA base pairs, which can be used for direct detection of mutations without any pre-screening. Its possible to do simultaneous screening of several genes for identification of allelic variants.
AgGenome can services offer coupled with other techniques like CRISPR
TILLING (Targeting Induced Local Lesions IN Genomes) is a reverse genetic technique that uses traditional chemical mutagenesis methods to create libraries of mutagenized individuals that are later subjected to high-throughput screens for the discovery of mutations. Previous TILLING protocols employed enzymatic or physical discrimination of heteroduplexed from homoduplexed target DNA. But these methods are very laborious, time consuming, needs extensive automation and sometimes has lower sensitivity and resolution than needed and the heteroduplexes need to be confirmed by Sequencing. AgriGenome offers mutation screening method based on Illumina sequencing of target genes amplified from multi-dimensionally pooled templates representing 768 individuals per experiment.