Bioreactors and Fermentors

Product Features

Low cost , compact, easy to use.
Multi-loop PID process control
Modular, upgradeable system
Autoclavable & in-situ systems
16-bit microprocessor controller
Configuration suits every budget

Vessel

Glass: Borosilicate glass vessels of volumes 2.5 L, 4 L, 7 L & 10L. Single walled and double-wall jacketed vessels available as per customer request. User defined volumes can also be supplied (f.e. 5L). Working volume is 40% to 90% of total volume (i.e. from 1L to 9L in different configurations). D / H ratio of vessel is 1:2 to 1: 3 depending on volume.

Stainless Steel:Stainless steel S316L vessels for volumes of 10 L and above. Standard volumes are 10 L, 15 L, 35 L. User defined sizes can be supplied too. Design pressure is 3 Bars.

Head Plate

Material: Stainless Steel 316L head plate has sufficient number of ports. Contact parts are made from SS316L. All ports have been designed to prevent contamination cross-over. Fitting of ports, sensors, tubings, motor etc. is convenient. Glass bottomed vessels have single top head-plate. Open-ended vessels have top head plate and bottom support plate.

Ports:Ports are provided on head plate and vessels made of SS 316L for pH sensor, DO sensor, foam sensor, temperature thermowell sensor, air sparger inlet, air venting exhaust with air filter, agitation motor or magnetic coupling stirrer shaft, heating/cooling finger for thermal control, acid, base/alkali, anti-foam addition, inoculum/septum feed and sample harvest, blind-plugged spare ports, drain port (in select models) etc. Contact parts are made from SS316L. All ports, valves, fittings have been designed to prevent contamination cross-over. Fitting of sensors, tubing, motor,etc is convenient. Ports have O-rings in pharma grade silicone.

Stirring & Agitation

Material: Stainless Steel 316L stirring rod with options for Rushton
type impellers, low-shear marine propellor type stirrer
blade or any user defined option. With baffles to prevent

vortex.

Coupling: Options for Top-driven Mechanical and Magnetic Coupling.

Magnetic :Magnetic coupling is top or bottom driven depending on user application. Magnetic coupling prevents
contamination with leakproof, sealless coupling between motor and stirrer shaft.Mechanical Mechanical lip seal with PTFE (Teflon) bush and rust-proof stainless steel bearing and o-rings & v-rings in pharma grade silicone; for contamination-free and sterile smooth agitation.

Motor :PMDC motor 80W high starting torque. AC motor can be provided in case user wishes so

Algorithm :16-bit Microprocessor PID Feedback control

Sensor :Gear tooth non-contact sensor for RPM measurement

Range: 100-1000 RPM with mechanical coupling. 50-500 RPM with magnetic coupling

Accuracy :Measurement and control accuracy of +/- 2 RPM

Display: Digital display of set and actual speeds on LCD or PC screen

General Construction

Control Panel Attractive control panel with LCD+keypad or optional
touch screen fitted

Parts: All valves (needle, ball etc), plugs, ports, fittings,connectors are SS316L grade. All rubber parts are pharma grade silicone.
Stand :Smaller models are desktop. In-situ models come withstands and frame to mount everything in compact lab
space

Datalogging: Windows software for datalogging and archiving controlled parameters

Interface :Via PC screen+keyboard/mouse or LCD touchscreen

Display: Displaying controlled parameters in a set of graphs on PC/laptop screen
Connectivity: Connection between PC/laptop and fermentor using RS232

Temperature

Material: Thermal transfer/exchanger finger made from SS316L for contact heating. For cooling, chiller unit with forced water or coolant circulation. TEM based solid state coolers canalso be provided. Chiller capacities range from 10L to 30L depending on vessel size

Algorithm: 16-bit Microprocessor PID Feedback control for heating. On-off feedback based auto control and cut-off of chiller for cooling.

Sensor :Platinum RTD Pt100 in SS316L thermowell

Range :3oC above room temperature to 60oC. Increments of 0.1oC. With water cooling system: 10oC to 60oC. Dependent on coolant and chiller capacity used sub-zero temperatures (down to -10oC) are also possible. Thermal insulating vessel blanket/cover will be provided for sub-zero temperatures.

Accuracy :Measurement and control accuracy of 0.1oC

Display :Digital display of set and actual temperature on LCD or PC screen

pH Module

Sensor :Autoclavable gel-filled glass pH electrode, sterilizable at 15 psi. Make: Broadley-James or Van-London-pHoenix, USA

Algorithm: 16-bit Microprocessor PID Feedback controlControl Microprocessor controls dual peristaltic pumps to dispense acid or alkali/base, automatically depending on set and actual pH. Two peristaltic pumps are standard

Range :Measurement range is 2 pH to 12 pH, increments of 0.1pH.

Accuracy :Measurement and control accuracy of +/- 0.1pH.

Display:Digital display of set and actual pH on LCD or PC screen

Peristaltic Pumps

Two fixed speed peristaltic pumps are with pH Control module. One fixed speed with Inoculum/Nutrient Feed/Harvest module for aseptic and contamination-free addition of media and removal of sample One fixed speed with Foam Control module So up to 4 pumps can be provided on the main control panel More peristaltic pumps can be provided as individual units Each peristaltic pump comes with its reservoir bottle and sterile port.
Aeration and DO Module Oxygen

Sensor :Autoclavable polarographic galvanic 12mm DO probe, sterilizable at 15 psi. Broadley-James or Van-London pHoenix, USA. Options for glass as well as stainless steel probes
Sensor Autoclavable polarographic galvanic 12mm DO probe,
sterilizable at 15 psi. Broadley-James or Van-London pHoenix,
USA. Options for glass as well as stainless steel
probes

Control :16-bit Microprocessor control. Two gases sparging through 0-5 LPM rotameters with needle valves are provided on front to control flow of air or gas manually

Range: Timer based sparging is standard. Optionally, control up to 100%, in increments of 1%.

Display :Digital display of set and actual values on LCD or PC screen

Filters: 0.2 um membrane sparger inlet and exhaust inline SS capsule filters to prevent contamination. Filters are
autoclavable to about 20 times

Compressor: Built-in oil-free diaphragm pump with push-fit connections and inline filters. Compact in size, makes low noise and suitable for biological and medical applications.

Sparger :Ring or straight tubular sparger with inline air filter and flexible silicone tubes.

Foam Control

Sensor: Autoclave-able stainless steel SS316L dual electrodes conductivity type, height-adjustable probe for foam
sensing

Control :16-bit Microprocessor control. Peristaltic pump switches on timed dispensing of anti-foam agent automatically in case of excess foam

Sterilization

Sterilizing inside autoclaves up to a pressure of 15 psi and 121oC. The vessel, head plate, impeller, probes, sparger, ports are all autoclavable. Optional in-situ sterilization with steam generator and solenoid valves. The in-situ sterilization system can be used for both SS and glass vessel bioreactors and fermenters.
Standard Configurations available in borosilicate glass vessels Even our basic units mentioned below are fully functional fermenters and bioreactors. They have been designed in such a way that the units meet your budget
yet at the same time are fully upgradeable in the future. So you can add advanced modules to increase functionality of basic systems. At the same time, the basic unit has systems to control motor speeds and agitation, motor RPM, temperature above ambient, will display pH values and has a 2-gas sparging and aeration system with filters and rotameters.
Model BIOF-01-01
Bioreactor basic unit with 1.5 litre working and 2.5 litre total volume.
Model BioF-01-02
Bioreactor basic unit with 3 litre working and 4 litre total volume.
Model BioF-01-03
Bioreactor basic unit with 5 litre working and 7 litre total volume. Basic unit has systems for · Agitation · Aeration · Temperature control (from 3C above room temp. to 60C) · pH display · Fermentor diagnostics (for remote technical
support).
Optional modules can be ordered · For temperatures below room temperature (Cooling System) · pH control (pH Control System) · control Dissolved Oxygen (DOdisplay and control system) · Foam Control · Feed & Harvest ·

Datalogging Software ·

Starter Kit · Extended Warranty Packs. (Order these with the basic unit of yourchoice)
Cooling system:
For attaining temperatures below room temperature. In case you need to set temperatures below your normal room temperatures, order this module. For example, if you need to run fermentation at 20C and your room temperature is
normally 30C, then order the cooling system.
pH Control system:
The basic unit has an autoclavable gel filled glass pH electrode that displays the real time pH of the run. If you need to control the pH of the reaction, use this module. It uses two peristaltic pumps with acid & base reservoirs which automatically add acid or base depending on the pH set and actual values.
Dissolved Oxygen Display & Control system:
With two gases sparging system. This module consists of a low-maintenance autoclavable glass DO electrode to measure and automatically sparge air to control the dissolved oxygen levels of the reaction.
Feed/Harvest Module:
With one bidirectional, variable speed peristaltic pump. Order this if you need to periodically add and remove media, inoculum and samples from the reactor vessel.
Foam Control Module:
With one peristaltic pump and conductivity probe that monitors the foam. Anti-foam agent is automatically added to the vessel in case of excess foam.
Data Logging Software:
MS Windows based software that displays graphs of and logs pH, temperature, DO
and RPM values to MS Excel sheets.