Transcriptome RNA Sequencing
Product Details:
RNA-Seq is a powerful approach that enables researchers to discover, profile and quantify RNA transcripts in the whole transcriptome. As this method does not use predesigned probes or primers, it becomes an unbiased hypothesis free approach providing researchers with applications that are not possible by traditional microarray based methods.RNA sequencing is used widely for:
Studies of gene expression and discovery of novel transcripts and isoforms
SNP discovery in the coding portions of the genome
Denovo transcriptome assembly
Basis for expression quantification in RNA-Seq
1- Counting or Profiling10 million total reads from poly-A selected RNA will give performance better than any microarray2- Studying Alternative Splicing or quantifying cSNPs for most transcriptsDeeper profiling of 50 to 100 million reads, with read lengths of 50 to 100bps, from poly-A selected3- Complete Annotation of an entirely New Transcriptome~500 Million reads of 100 bp read length from multiple tissues