Clonesaver Card

Product Details :

FTA Technology in 96 well format for high-throughput applications. Designed for the collection, long-term storage and purification of plasmid and BAC DNA from bacterial clones in a 96 well format.

Prepare BAC and plasmid DNA with amazing ease :

• Apply 5 µl bacterial culture, resuspended colony or glycerol stock. Cells are lysed and plasmid or BAC DNA is stabilized for long-term storage or immediate processing.
• DNA is easily accessible for downstream applications
• Store up to 96 samples on each card

Store sample DNA for years at room temperature

Plasmid DNA stored on CloneSaver Cards is stable at room temperature for at least four years...and counting.

DNA is Easily Accessible for Downstream Applications:

Transformation

Plasmid DNA can be eluted or used directly on a punch to transform bacteria either by electroporation or heat-shock methods.

PCR

Immobilized plasmid DNA on a CloneSaver Card punch can be used directly in a PCR. The PCR products remain in solution, do not bind to the punch, and are easily recoverable. Plasmid DNA can also be eluted for PCR or other studies.

Sequencing

Plasmid DNA eluted from a CloneSaver punch can be amplified by Rolling Circle Amplification, such as TempliPhi™ from GE Healthcare (formerly Amersham Biosciences) and then sequenced without the need for culture regrowth and plasmid purification.

Clone Saver Resealable Multi-Barrier Pouches

Used for transporting or storing the CloneSaver Card. The pouch is constructed with seven laminated layers that protect the card from exposure to gas or liquid contamination. There is a zip-lock resealable closure for easy access to the CloneSaver Card. The tamper-evident seal maintains sample security and the outer paper surface can be used for labeling or writing.

SPOT CloneSaver Holder

SPOT CloneSaver Holder is a rigid frame that allows automated spotting to standard CloneSaver Cards. It keeps the card flat for uniform and precise spotting of biological samples. The 96 well card is easily inserted into the ANSI/SBS-compatible frame, which can then be placed onto a liquid-handling deck just like a multiwell plate.

The SPOT CloneSaver Holder is compatible with standard liquid handlers manufactured by companies such as Beckman Coulter and Tecan Instruments.

CloneSaver Starter Kit

Includes: 2 CloneSaver Cards, 2 Uni-Core Punches (2 mm) with cutting mat, instructions.

Additional Information:

• Delivery Time: As per location
• Packaging Details: Packet